Journal: Molecular Medicine Reports

Article Title: Collagen III regulates the termination of liver regeneration by suppressing hepatocyte proliferation and promoting functional recovery

doi: 10.3892/mmr.2026.13799

Figure Lengend Snippet: Protein and mRNA expression of collagen III after PHx in mice. (A) IF staining of collagen III. Scale bar, 50 µm (×200 magnification) and 20 µm (×400 magnification). (B) Relative fluorescence intensity of collagen III in parenchymal areas. (C) Reverse transcription-quantitative PCR analysis of Col3a1 mRNA levels. *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001. PHx, partial hepatectomy; col3a1, collagen III α1.

Article Snippet: Collagen III , Proteintech Group, Inc. , 22734-1-AP , IF , 1:300.

Techniques: Expressing, Staining, Fluorescence, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Molecular Medicine Reports

Article Title: Collagen III regulates the termination of liver regeneration by suppressing hepatocyte proliferation and promoting functional recovery

doi: 10.3892/mmr.2026.13799

Figure Lengend Snippet: Liver regeneration status after collagen III degradation during the termination phase of liver regeneration. (A) Body weight of mice. (B) Liver-to-body weight ratio of mice. (C) Dual IF staining of EdU and Ki-67. (D) Dual IF staining of EdU and collagen III. Scale bars, 100 and 10 µm. *P<0.05, **P<0.01 and ***P<0.001. ns, not significant; PHx, partial hepatectomy; IF, immunofluorescence; Coln0.1, low-dose collagenase III; Coln0.2, high-dose collagenase III; Col III, collagen III.

Article Snippet: Collagen III , Proteintech Group, Inc. , 22734-1-AP , IF , 1:300.

Techniques: Staining, Immunofluorescence

Journal: Molecular Medicine Reports

Article Title: Collagen III regulates the termination of liver regeneration by suppressing hepatocyte proliferation and promoting functional recovery

doi: 10.3892/mmr.2026.13799

Figure Lengend Snippet: Inhibition of β-catenin partially rescues impaired regeneration termination and liver dysfunction induced by collagen III degradation. (A) Schematic diagram of the animal experimental protocol; created with BioGDP.com (agreement no. GDP2025X2AT2F) . (B) WB analysis of β-catenin after collagen III degradation. (C) Body weight and (D) liver-to-body weight ratio of mice following collagen III degradation and β-catenin inhibition. (E) Dual immunofluorescence staining of EdU and collagen III following collagen III degradation and β-catenin inhibition. Scale bars, 100 and 10 µm. (F) Serum liver function marker levels following collagen III degradation and β-catenin inhibition. (G) WB analysis of HNF4α following collagen III degradation and β-catenin inhibition. (H) WB analysis of cyclin D1 following collagen III degradation and β-catenin inhibition. *P<0.05, **P<0.01 and ***P<0.001. ns, not significant; WB, western blot; PHx, partial hepatectomy; MSAB, methyl-sulfonyl AB; Coln0.1, low-dose collagenase III; Coln0.2, high-dose collagenase III; Col III, collagen III; ALT, alanine aminotransferase; HNF4α, hepatocyte nuclear factor 4α.

Article Snippet: Collagen III , Proteintech Group, Inc. , 22734-1-AP , IF , 1:300.

Techniques: Inhibition, Immunofluorescence, Staining, Marker, Western Blot

Journal: ACS Applied Materials & Interfaces

Article Title: Nanohydroxyapatite Coating Attenuates Fibrotic and Immune Responses to Promote Keratoprosthesis Biointegration in Advanced Ocular Surface Disorders

doi: 10.1021/acsami.4c04077

Figure Lengend Snippet: Corneal tissue fibrotic responses to the AuroKPro implantation. The responses were visualized and quantified with immunohistochemical analysis testing for four markers of fibrosis: fibronectin (A), CD90 (B), collagen 3A1 or COL3A1 (C), and α-smooth muscle actin or α-SMA (D). In chemically injured corneas, all markers, except CD90, were expressed in the tissue adjacent to the PMMA optical cylinders at a significantly higher level following implantation of noncoated KPros relative to nHAp-coated KPros. In noninjured situations, all fibrosis markers appeared greater with the noncoated KPros but the differences were not statistically significant. P indicates the space vacated by the PMMA optical cylinder. Scale bars = 50 μm. Data are presented as mean ± SE ( n = 4 in each group). * p < 0.05. NI = noninjured eyes. CI = chemically injured eyes. NC = noncoated. C = coated.

Article Snippet: The sections were washed in 1× PBS (first BASE), blocked in 4% BSA (Sigma-Aldrich), and incubated with mouse monoclonal antibodies against cellular fibronectin (clone DH1, Sigma-Aldrich), α-SMA (clone 1A4, Agilent Technologies, Santa Clara, California), and CD90 (clone OX7, Santa Cruz Biotechnology) and goat polyclonal antibody against collagen type IIIA1 (COL3A1) (Southern Biotech, Birmingham, Alabama) overnight.

Techniques: Immunohistochemical staining

Journal: Journal of Translational Medicine

Article Title: Cryopreservation of human vascular umbilical cord cells under good manufacturing practice conditions for future cell banks

doi: 10.1186/1479-5876-10-98

Figure Lengend Snippet: Expression of cellular marker molecules and extracellular matrix (ECM) proteins by human umbilical cord artery derived cells (HUCAC). Using indirect immunofluorescence staining, highly positive signals (green) were detected for A ) collagen type I of fresh cultivated cells and B ) collagen type I of cryopreserved cells, E ) collagen type III of fresh cultivated cells and F ) collagen type III of cryopreserved cells. The presence of C ) collagen type I (green) and G ) collagen type III (green) was shown in native human umbilical cord artery walls, serving as a control. Immunohistochemical staining verified the presence of D ) collagen type I (red) and H ) collagen type III (red) in native human umbilical cord artery walls. Using flow cytometry analysis, cellular marker expression of short-term (group A, n = 4) and long-term (group B, n = 4) cryopreserved cells from primary cultures (passage 0) was studied directly after I ) thawing and J ) in passage 3 of recultivation. By comparison, non-cryopreserved fresh cells (n = 3) from I ) primary cultures and J ) passage 3 were analyzed in parallel as a control group Using indirect immunofluorescence staining, highly positive signals (green) were detected for all cellular markers tested such as K ) CD90 (green)/ alpha smooth muscle actin (ASMA) (red) of fresh cultivated cells and L ) CD90 (green)/ ASMA (red) of cryopreserved cells, N ) CD29 of fresh cultivated cells and O ) CD29 of cryopreserved cells, P ) CD105 of fresh cultivated cells and Q ) CD105 of cryopreserved cells. Cell nuclei staining is pictured in blue, present in A-H and K-Q. All studies of marker expression are exemplarily shown for cells of passage 3.

Article Snippet: Fixed cross sectioned umbilical cord arteries were used to localize HUCAC and served as controls by staining with monoclonal mouse anti-human CD90 (4 μg/ml, Dianova), anti-human fibronectin (5 μg/ml, BD Biosciences) and with polyclonal rabbit anti-human ASMA (25 μg/ml, Abcam), anti-human collagen types I & III (0.5 μg/ml, Acris Antibodies), anti-human elastin (1:50, Calbiochem) for 3 h at 4°C.

Techniques: Expressing, Marker, Derivative Assay, Immunofluorescence, Staining, Control, Immunohistochemical staining, Flow Cytometry, Comparison